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. Author manuscript; available in PMC: 2014 Jan 24.
Published in final edited form as: Chem Biol. 2013 Jan 24;20(1):10.1016/j.chembiol.2012.10.020. doi: 10.1016/j.chembiol.2012.10.020

Figure 3. Sorting of the DhbE Library Displayed on the Yeast Cell Surface by Binding to Biotin-Linked 3-HBA-AMS 9.

Figure 3

(A) Sorting with magnetic beads coated with streptavidin. Binding of cells to 9 and an anti-Myc antibody was analyzed by flow cytometry.

(B) FACS sorting by antibody binding to the HA and Myc tags flanking DhbE on the cell surface.

(C–E), FACS by binding to 9 and an anti-Myc antibody. Percentages of doubly labeled cells were shown in the flow cytometry plots. Red frames in the plots represent the sorting gates used for the collection of yeast cells.