Figure 3. Sorting of the DhbE Library Displayed on the Yeast Cell Surface by Binding to Biotin-Linked 3-HBA-AMS 9.
(A) Sorting with magnetic beads coated with streptavidin. Binding of cells to 9 and an anti-Myc antibody was analyzed by flow cytometry.
(B) FACS sorting by antibody binding to the HA and Myc tags flanking DhbE on the cell surface.
(C–E), FACS by binding to 9 and an anti-Myc antibody. Percentages of doubly labeled cells were shown in the flow cytometry plots. Red frames in the plots represent the sorting gates used for the collection of yeast cells.