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. Author manuscript; available in PMC: 2014 Jan 24.
Published in final edited form as: Chem Biol. 2013 Jan 24;20(1):10.1016/j.chembiol.2012.10.020. doi: 10.1016/j.chembiol.2012.10.020

Figure 4. MALDI Analysis of Transfer of 3-HBA Substrate to the ArCP Domain Catalyzed by wtDhbE and the KZ4 Mutants.

Figure 4

(A–C) Reactions were allowed to proceed for 30 min at room temperature. (A) wtDhbE has some activity of loading 3-HBA to the ArCP domain of DhbB. (B) KZ4 mutant is defective in 3-HBA loading to ArCP. (C) The KZ4 (Trp234His) mutant can efficiently load 3-HBA to ArCP.