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. Author manuscript; available in PMC: 2014 Jan 24.
Published in final edited form as: Chem Biol. 2013 Jan 24;20(1):10.1016/j.chembiol.2012.10.020. doi: 10.1016/j.chembiol.2012.10.020

Figure 5. Kinetic Analysis of DHB and 3-HBA Transfer to ArCP by wtDhbE and KZ4 (Trp234His) Based on the PPi Release Assay.

Figure 5

Michaelis-Menten plot to compare the initial rates of PPi release catalyzed by wtDhbE and KZ4(Trp234His) at varying concentrations of native substrate DHB (A) and nonnative substrate 3-HBA (B). Data are means ± ranges for at least three determinations.