Figure 2.

Subcellular Localization of PRORP1 and PRORP2

Analysis of the subcellular localization in procyclic T. brucei Lister 427 cells by expression of C-terminally YFP-tagged proteins (YFP) or by immunofluorescence (IF) of endogenous proteins is presented. Mitochondria were stained by MitoTracker red (MT), nuclei and kinetoplasts by Hoechst (H); cells are also shown in bright-field view (BF). Pictures were taken by epifluorescence microscopy. Scale bars, to 2 μm.

(A) Expression of YFP-tagged PRORP1 is shown.

(B) Immunofluorescence of endogenous PRORP1 is demonstrated; overlay of immunofluorescence and Hoechst staining (IF/H) is illustrated.

(C) Expression of YFP-tagged PRORP2 is shown.

(D) Immunofluorescence of endogenous PRORP2 is demonstrated.