FIG. 4.

Angiogenic secretome profiles of differentiated ECs and SMCs, and their paracrine interaction. (A) The angiogenesis proteome profiler array system was used to screen the cytokines and growth factors in the CM harvested from ECs and SMCs. The boxes on membranes marked EGF (right boxes) and VEGF (left boxes). (B) mRNA levels of VEGF, EGF, and FGF-4 in SES8-ECs, H9-ECs, and HUVECs were analyzed using RT-PCR. (C) Quantification of the number of migrated SMCs. SMCs were placed in the upper transwell chamber and the CM harvested from ECs was added to the lower chamber (mean±SEM, *p<0.05 vs. HUVEC, n=4). CM, conditioned medium; EGF, epidermal growth factor; VEGF, vascular endothelial growth factor; FGF, fibroblast growth factor. Color images available online at www.liebertpub.com/tea