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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Mol Microbiol. 2013 Jul 29;89(5):10.1111/mmi.12325. doi: 10.1111/mmi.12325

Figure 4.

Figure 4

The effect of QrrU27G mutation on the expression of Vca0939-Flag in V. cholerae. V. cholerae strains expressing the indicated plasmid-borne qrr2 and vca0939-flag alleles were grown and analyzed for protein production using western blot. Liquid cultures of the strains indicated were pelleted, resuspended in sample buffer, subjected to SDS-PAGE, and transferred to PVDF membrane. Blots were probed with an antibody to the Flag tag and visualized. Shown is one representative of three experiments performed.