Table 1.
Strains and plasmids used in this study
| Strain | Description | Reference |
|---|---|---|
| BH2126 | V. choleraeΔqrr1–4, luxOD47E | Hammer & Bassler, 2007 |
| BH2130 | E. coli for 5’RACE | Hammer & Bassler, 2007 |
| Plasmids used in E. coli | Description | Reference |
| pEVS141 | vector control for pQrr2Ec | (Dunn et al., 2006) |
| pQrr2Ec | Ectopic WT Qrr2 expression | Bardill et al., 2011 |
| pQrr2U27CEc | Ectopic Qrr2U27C expression | This study |
| pKK | vector control for pVca0939Ec | Lenz et al., 2004 |
| pVca0939Ec | Vca0939-FLAG reporter | This study |
| pVca0939A43CEc | Vca0939A43C-FLAG reporter | This study |
| pVca0939ΔLSEc | Vca0939ΔLS -FLAG reporter | This study |
| pVca0939ΔRSEc | Vca0939ΔRS -FLAG reporter | This study |
| pVca0939ΔLS+RSEc | Vca0939ΔLS+RS -FLAG reporter | This study |
| pVca0939C41GEc | Vca0939C41G-FLAG reporter | This study |
| pVca0939G44CEc | Vca0939G44C-FLAG reporter | This study |
| pVca0939G35CEc | Vca0939G35C-FLAG reporter | This study |
| Plasmids used in V. cholerae | Description | Reference |
| pKK | vector control for pQrr2 | Lenz et al., 2004 |
| pQrr2 | Ectopic WT Qrr2 expression | This study |
| pQrr2U27C | Ectopic Qrr2U27C expression | This study |
| pEVS141 | vector control for pVca0939 | Dunn et al., 2006 |
| pVca0939 | Vca0939-FLAG reporter | This study |
| pVca0939A43C | Vca0939A43C-FLAG reporter | This study |
| pVca0939AAEEF | Vca0939AAEEF-FLAG reporter | This study |
| pVca0939GGEAF | Vca0939GGEAF-FLAG reporter | This study |
| Other Plasmids | Description | Reference |
| pPB004 | Qrr2 in vitro transcription | Bardill et al., 2011 |
| pEZ637 | Vca0939 in vitro transcription | This study |
| pEZ731 | Qrr2U27Gin vitro transcription | This study |
| pEZ774 |
Vca0939A43C in vitro transcription |
This study |
| pPB007 | Hfq expression plasmid | Bardill et al., 2011 |