Fig 6.

Real-time imaging of BDV intracellular traffic in Vero cells. (A to C) Visualization of viral transfer between two infected Vero cells. Persistently infected Vero cells were FlAsH labeled and imaged for 10 h by confocal microscopy. Images were acquired every 4 min. FlAsH-labeled viral material (arrow) was transmitted through a cytoplasmic extension of a neighboring cell and was progressively routed toward the nucleus of the cell shown. (D to F) Visualization of BDV traffic during early stages of infection. Noninfected Vero cells transfected with an mRFP-Cherry expression plasmid (red) were cocultivated with FlAsH-labeled BDV-infected Vero cells (green). Thereafter, this coculture was imaged by confocal microscopy for 6 h. Images were acquired every 4 min. The arrow points to viral material that was transmitted to the noninfected cell and that was progressively routed toward its nucleus throughout the observation period. Bar = 10 μM.