Fig 7.

Cytoskeleton rearrangement is not essential for BDV cell-to-cell spread. Vero cells persistently infected by rBDV-P-Flag-TCT were labeled with FlAsH and treated with 5 μM cytochalasin D throughout live-cell imaging. (A) Validation of cytochalasin D impact on the cytoskeleton network. Cells were treated for different times (1 to 6 h, results being identical in all cases) and labeled with Alexa 488-conjugated phalloidin. Bar = 20 μm. (B and C) Two representative examples of cell-to-cell BDV spread using cytochalasin D-treated, FlAsH-labeled BDV-infected Vero cells. These cultures were imaged by confocal microscopy for 6 h. Images were acquired every 4 min. The arrows point to viral material that was transmitted between cells and that was progressively routed toward the nucleus throughout the observation period. Bar = 10 μm.