Table 1.
Bacterial strains and plasmids used in this work
| Strain or plasmid | Relevant characteristics |
|---|---|
| Y. pestis strain | |
| 201 (wild type) | Avirulent to humans, highly lethal to mice |
| 201pKD46 | 201 harboring pKD46 vector, Ampr |
| 201ΔwaaA(YPO0055) | ΔwaaA, derived from strain 201pKD46, Kmr cassette replacement of waaA gene |
| 201ΔlamB(YPO3711) | ΔlamB, derived from strain 201pKD46, Kmr cassette replacement of lamB gene |
| 201ΔompF(YPO1411) | ΔompF, derived from strain 201pKD46, Kmr cassette replacement of ompF gene |
| 201ΔompA(YPO1435) | ΔompA, derived from strain 201pKD46, Kmr cassette replacement of ompA gene |
| 201Δail(YPO2905) | Δail, derived from strain 201pKD46, Kmr cassette replacement of ail gene |
| 201C-lamB | Complemented ΔlamB strains, transformed with a pACYC184 vector with cloned lamB |
| 201C-ompF | Complemented ΔompF strains, transformed with a pACYC184 vector with cloned ompF |
| 201C-ompA | Complemented ΔompA strains, transformed with a pACYC184 vector with cloned ompA |
| 201C-ail | Complemented Δail strains, transformed with a pACYC184 vector with cloned ail |
| 201C-waaA | Complemented ΔwaaA strains, transformed with a pACYC184 vector with cloned waaA |
| 201ΔailΔompA | Δail ΔompA, derived from strain 201ΔompA, Cmr cassette replacement of ail gene |
| 201ΔlamBΔail | ΔlamB Δail, derived from strain 201Δail, Cmr cassette replacement of lamB gene |
| 201ΔompFΔail | ΔompF Δail, derived from strain 201Δail, Cmr cassette replacement of ompF gene |
| 201ΔompFΔompA | ΔompF ΔompA, derived from strain 201ΔompA, Cmr cassette replacement of ompF gene |
| 201ΔompFΔlamB | ΔompF ΔlamB, derived from strain 201ΔlamB, Cmr cassette replacement of ompF gene |
| 201ΔompAΔlamB | ΔompA ΔlamB, derived from strain 201ΔlamB, Cmr cassette replacement of ompA gene |
| 201ΔompFΔailΔompA | ΔompF Δail ΔompA, derived from strain 201ΔailΔompA, Gmr cassette replacement of ompFgene |
| 201ΔailΔompAΔlamB | Δail ΔompA ΔlamB, derived from strain 201ΔompAΔlamB, Gmr cassette replacement of ail gene |
| 201ΔompFΔompAΔlamB | ΔompF ΔompA ΔlamB, derived from strain 201ΔompAΔlamB, Gmr-cassette replacement of ompF gene |
| 201ΔailΔompFΔwaaA | Δail ΔompF ΔwaaA, derived from strain 201ΔompFΔail, Gmr cassette replacement of waaA gene |
| Y. pseudotuberculosis | Pa3606 Human, Japan Yp 1b |
| Plasmids | |
| pKD46 | Red recombinase plasmid; Ampr |
| pET28a::gp17 | Expression vector with a PCR-amplified gp17 gene in the NheI/HindIII site; Kmr |
| pET28a::lamB | Expression vector with a PCR-amplified lamB gene in the NheI/HindIII site; Kmr |
| pET32a::ompF | Expression vector with a PCR-amplified ompF gene in the BamHI/SalI site; Ampr |
| pET32a::ompA | Expression vector with a PCR-amplified ompA gene in the BamHI/SalI site; Ampr |
| pET32a::ail | Expression vector with a PCR-amplified ail gene in the BamHI/SalI site; Ampr |
| pACYC184::waaA | Cloning vector with a PCR fragment covering a region from 500-bp fragment upstream to 300-bp fragment downstream of the waaA gene in the BamHI/HindIII site;Cmr |
| pACYC184::lamB | Cloning vector with a PCR fragment covering a region from 500-bp fragment upstream to 300-bp fragment downstream of the lamB gene in the BamHI/HindIII site; Cmr |
| pACYC184::ompF | Cloning vector with a PCR fragment covering a region from 500-bp fragment upstream to 300-bp fragment downstream of the ompF gene in the BamHI/HindIII site; Cmr |
| pACYC184::ompA | Cloning vector with a PCR fragment covering a region from 500-bp fragment upstream to 300-bp fragment downstream of the ompA gene in the BamHI/HindIII site; Cmr |
| pACYC184::ail | Cloning vector with a PCR fragment covering a region from 500-bp fragment upstream to 300-bp fragment downstream of the ail gene in the BamHI/HindIII site; Cmr |