Fig 2.

Replication kinetics of enteroviruses in human brain-derived cell lines. Subconfluent monolayers of CCF-STTG1, T98G, and SK-N-SH cells were infected with enteroviruses at an MOI of 1 in 24-well plates. At various times after infection, the virus titers in the culture supernatant (extracellular virus) for all three cell lines tested and the cell-associated fraction (intracellular virus) for CCF-STTG1 cells were determined by the TCID₅₀ assay. The results are presented as the means ± standard deviations obtained from three independent experiments.