Fig 6.

Cell morphology change, viral antigen detection, and virus yield following RNA transfection. (A) CCF-STTG1 cells were transfected with CVB3 genomic RNA or RNA upon treatment with RNase. An extensive cytopathic effect was observed in the cultures on day 1 following RNA transfection, and high percentages of cells (40%) were shown to be CVB3 antigen positive (green) by IFA. Nuclei were counterstained with Hoechst 33258 (blue). Magnification, ×100. The results of one representative experiment out of three are shown. (B) At the indicated times posttransfection, samples of supernatant were harvested and virus titers were determined. The means and standard deviations of three independent experiments are shown.