Table 1.
Oligonucleotides used in this study
| Name | Nucleotide sequence (5′→3′)a | Genome positionb |
|---|---|---|
| Pos-Fwd | TAATACGACTCACTATAGGTCTGTTACCCCGGACTGAGT | 173–192 |
| Pos-Rev | GCTCTATTGGTCACCGGATG | 653–634 |
| Neg-Fwd | TAATACGACTCACTATAGGGCTCTATTGGTCACCGGATG | 653–634 |
| Neg-Rev | TCTGTTACCCCGGACTGAGT | 173–192 |
| Tag-FP | CGGTCATGGTGGCGAATAACAGACATGGTGCGAAGAGTCT | 407–427 |
| Tag-RP | CGGTCATGGTGGCGAATAAAGTAGTCGGTTCCGCTGCA | 547–529 |
| RP | AGTAGTCGGTTCCGCTGCA | 547–529 |
| FP | AGACATGGTGCGAAGAGTCT | 407–427 |
| Probe | FAM-TAGTCCTCCGGCCCCTGAATGC-TAMRA | 444–465 |
| Tag | CGGTCATGGTGGCGAATAA |
a
The T7 promoter sequence is shown in boldface. The nonenterovirus Taq sequence is indicated in italics. FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine.
b
Relative to CVB3 strain Nancy.