Table 1.
Primers used for RT-qPCR and ChIP-qPCR
| Method and primera | Orientation | Sequence (5′-3′) |
|---|---|---|
| RT-qPCR, cDNA | ||
| PIG3 | Forward | GCTTCAAATGGCAGAAAAGC |
| Reverse | AACCCATCGACCATCAAGAG | |
| HPV38 E6 | Forward | TCTGGACTCAAGAGGATTTTG |
| Reverse | CACTTTAAACAATACTGACACC | |
| HPV38 E7 | Forward | CAAGCTACTCTTCGTGATATAGTT |
| Reverse | CAGGTGGGACACAGAAGCCTTAC | |
| GAPDH | Forward | AAGGTGGTGAAGCAGGCGT |
| Reverse | GAGGAGTGGGTGTCGCTGTT | |
| ChIP-qPCR, gene promoters | ||
| PIG3 (−240/−399) | Forward | CCCAGGACTGCGTTTTGCCT |
| Reverse | GGTCCATTTTCCAGGCATGG | |
| PIG3 (+322/+517) | Forward | CAGGAGCTCGGGGCGGA |
| Reverse | ACAGGGCAGGGCAGGGC | |
| Survivin | Forward | TGGGTGCCCCGACGT |
| Reverse | GAAGGGCCAGTTCTTGAATGTAGA | |
| FasL | Forward | GATCCCGCTGGGCAGGC |
| Reverse | GTTCTGAAGGCTGCAGGCTC | |
| p21waf1 | Forward | TCACCATTCCCCTACCCCATGCTGCTC |
| Reverse | AAGTTTGCAACCATGCACTTGAATGTG | |
| CaN19 | Forward | GGTCCAGGATGCCCAGTC |
| Reverse | GAAGGAGAGCAAGGCAGC | |
| GAPDH | Forward | CACTGGAGCCTTCATCTCAG |
| Reverse | CTGTTGCTGGCCAGCAACTG |
a
The region is indicated in parentheses where applicable.