Table 2.
Primers used for PCR and pyrosequencing
| Primera | Sequence (5′–3′) |
|---|---|
| PIG3 pyro (−566/−458) | |
| Forward | GGTATGGTTAGGTTTTTGGT |
| Reverse | CCAAAAATACTACTAAACATCCTAC |
| Seq1 | GGAGAAGGATGTTGG |
| Seq2 | AAGGTTTTTTAATTT |
| Seq3 | TGGATTAGTTAGTT |
| PIG3 pyro (+97/+103) | |
| Forward | GTTGGATGYGGTAGAGTAGGAT |
| Reverse | TTCTACTTAACCCCACCCC |
| Seq1 | TGGAAGTTTTTAGT |
| Seq2 | TTTTTATGTATTAGG |
| Seq3 | GGTTTTGGTTTTTG |
| PIG3 pyro (+339/+359) | |
| Forward | GAGGGAGTGTGATTTGTTTG |
| Reverse | CAACCCAACCTCAAACTAAC |
| Seq1 | TTTGATTTTTTT |
| Seq2 | TATTTTATTTTT |
| Seq3 | TAGGTAGGAGTT |
a
The region is indicated in parentheses where applicable. Seq, sequencing.