Fig 10.

Depletion of Refrex-1 from cell culture supernatants. Culture supernatants from HEK293T, HEK293T expressing ERV-DC7 Env (HEK293T/DC7), HEK293T expressing ERV-DC16 Env (HEK293T/DC16), and the feline cell lines 3201, AH927, and CRFK were treated with goat anti-FeLV gp70 antibody or normal goat serum (control), and Refrex-1 was depleted from the supernatants. The remaining supernatants were diluted with medium, and 250 μl of each supernatant was added to the culture when HEK293T cells were infected with FeLV-D/Ty26 pseudotyped virus. X-Gal-positive cells were counted as infectious units (I.U.) at 48 h postinfection.