Fig 2.

Viral interference assay of LacZ pseudotyped viruses. (A) Western blot analysis with anti-FeLV Env (gp70) antibody in HEK293T cells transfected with env expression plasmids. Anti-actin antibody was also used as a control. DC18 infection; HEK293T cells were infected with a replication competent infectious virus of ERV-DC18. (B) env genes of FeLV-A/Glasgow-1, FeLV-B/Gardner–Arnstein, FeLV-C/Sarma, FeLV-D/Ty26, FeLV-D/ON-T, FeLV-D/ON-C, FeLV-D/44B, ERV-DC6, ERV-DC8, ERV-DC10, ERV-DC14, ERV-DC19, and A-MLV (Ampho-MLV/4070A) were used for preparation of LacZ pseudotyped viruses. HEK293T cells preinfected with no virus (gray), FeLV-A/clone33 (red), FeLV-B/Gardner–Arnstein (GA; green), FeLV-C/Sarma (purple), FeLV-D (FeLV-D/c33 chimeric virus; aqua blue), or ERV-DC10 (orange) were used as target cells for the interference assay. X-Gal-positive cells were counted as infectious units (I.U.) at 48 h postinfection.