Fig 3.

Viral infection assay of lacZ pseudotyped virus in the presence of supernatant of 3201 cells or FeLIX. (A) env genes from Ampho-MLV/4070A (A-MLV/4070A), FeLV-D/Ty26, ERV-DC10, and FeLV-T/NS33-4 were used for pseudotyped virus preparation. HEK293T cells were used as target cells for the viral infection assay. The supernatant of 3201 cells (3201 Sup.; blue) or HEK293T cells transfected with FeLIX expression vector (green) or empty vector (Mock; red) was added in the culture when HEK293T cells were infected with each pseudotyped virus. (B) Dose-dependent inhibition by the supernatant of 3201 cells in the viral infection assay. Supernatant of 3201 cells was added to the culture and tested for infection using FeLV-D/Ty26 pseudotyped virus. X-Gal-positive cells were counted as infectious units (I.U.) at 48 h postinfection.