Table 1.
Expression plasmids constructed for this studya
| Expression plasmid | Template plasmid | Forward primer | Reverse primer |
|---|---|---|---|
| pFUΔss ON-C | p44B peL | Fe-130S | Fe-50R |
| pFUΔss 44B | pON-C | Fe-130S | Fe-50R |
| pFUΔss DC6 | pCR4 ERV-DC6 | Fe-130S | Fe-126R |
| pFUΔss DC8 | pCR4 ERV-DC8 | Fe-130S | Fe-126R |
| pFUΔss DC14 | pCR4 ERV-DC14 | Fe-130S | Fe-126R |
| pFUΔss DC19 | pCR4 ERV-DC19 | Fe-130S | Fe-126R |
| pFUΔss 4070A | pCL-Ampho | 4070A-2S | 4070A-2R |
| pFUΔss FeLIX | pCR-Blunt FeLIX | FeLIX-1F | FeLIX-1R |
| pFUΔss 3201-2D | pCR-Blunt 3201-2D | Fe-207S | Fe-190R |
| pFUΔss 3201-2A | pCR-Blunt 3201-2A | Fe-209S | Fe-187R |
a
Each DNA fragment was PCR amplified with the indicated primers and template plasmid and then inserted into the pFUΔss expression vector. Primer sequences are shown in Table 2.