Table 2.
Sequences of primers used in this study
| Primer | Sequence (5′–3′)a | Cloning site |
|---|---|---|
| Fe-44S | CGGAATTCATCGAGATGGAAGGTCC | EcoRI |
| Fe-130S | CCGAATTCTRCACAAACCCAAAATGA | EcoRI |
| Fe-184S | CCTAGGRGCTTGGBTCCYAACATTTGGTG | |
| Fe-205S | GGATCCGGATCCATGAAACCCCCAGCGGGAAT | BamHI |
| Fe-206S | GGATCCGGATCCATGAAACCCCCAACAGGAAT | BamHI |
| Fe-207S | GGATCCGGATCCATGAAACCCCCAACAGGAAT | BamHI |
| Fe-208S | GGATCCGGATCCATGAGACCCTCAGCAAGAAT | BamHI |
| Fe-209S | GGATCCGGATCCATGAAACTCCCAACAGGAAT | BamHI |
| Fe-219S | GCCACGGTCATGAAAATAAAAA | |
| FeLIX-1F | CTGGATCCATGGAAGGTCCAACGCACCCAA | BamHI |
| 4070A-2S | TCTGGCTAGCCATGGCGCGTTCAACGCTCTCAAA | NheI |
| Fe-44R | TGCAGACAGAACATACTGTGACAAA | |
| Fe-50R | TTGAATTCTCATGGTTGGTCTGGATCGTATTG | EcoRI |
| Fe-126R | CAGAATTCTCTCATTCCCCCATTTTCTTT | EcoRI |
| Fe-168R | GAAGRTAGGGTGGGGGTGTKTTAGTAAGCTA | |
| Fe-184R | GAATTCGAATTCTATTCGATTGTATCTGGCCTTT | EcoRI |
| Fe-185R | GAATTCGAATTCTTTAGGAGCCTATCTCCTGT | EcoRI |
| Fe-186R | GAATTCGAATTCTCATTCAATTGTATCTGGCCTTTCTG | EcoRI |
| Fe-187R | GGATCCGGATCCTCAAGAGGGGGAAGTTGAGTATC | BamHI |
| Fe-188R | GGATCCGGATCCTTAGGAGTCTGTCTCCCGTG | BamHI |
| Fe-190R | GGATCCGGATCCTCAGGTTGGGCCTACGGTTTGGG | BamHI |
| Fe-196R | GGATCCGGATCCTTAGGAGTCTGTCTCCCGTG | BamHI |
| FeLIX-1R | TAGAATTCTTAGCTGGGGTGAGGTATTACT | EcoRI |
| 4070A-2R | TTATGCTAGCTATGGCTCGTACTCTATAGGCT | NheI |
a
Restriction enzyme sites are underlined. The indicated enzyme site was used for the construction of the expression vector shown in Table 1.