Conservation of the zinc binding structure at the β annulus. (A) The N termini of the C subunit around the putative zinc binding site for CNV (red) and TBSV (pink). The mauve spheres represent the zinc ions. Not only is the position of H62 highly conserved, but the site that affects transmission, P73, is also conserved. (B) Sequence alignment of this region in Cymbidium ring spot virus (CyRSV; a tombusvirus), Turnip crinkle virus (TCV; a carmovirus), Melon necrotic spot virus (MNSV; a carmovirus), Red clover necrotic mosaic virus (RCNMV; a dianthovirus), Cucumber leaf spot virus (CLSV; an aureusvirus), and Pathos latent virus (PoLV; an aureusvirus).