Fig 6.

(A) Locations of mutations causing defective transmission in CNV isolated via mechanical passaging. Shown here is the C subunit of CNV, with the mutation sites denoted by spheres. The orientation here is with the RNA at the bottom and the icosahedral 3-fold axis running vertically on the right side. The various mutations and their effects are shown in Table 2. (B) Site-directed mutagenesis of CNV around the locations of the naturally occurring mutants. The various mutations and their effects are summarized in Table 3. Red asterisks, the three sites where mutations affected transmission, but not particle formation.