Table 3.
Site-directed mutagenesis of the capsid protein and effects on particle assembly and transmission
| Mutation | Location | Particle assembly | % transmissiona |
|---|---|---|---|
| Wild type | + | 87 (34/39) | |
| P63G | Arm | + | 10 (2/20) |
| P63A | Arm | + | 100 (5/5) |
| P67G | Arm | + | 100 (5/5) |
| P67A | Arm | + | 100 (4/4) |
| P73G | Arm | + | 4 (1/25) |
| P85A | Arm | + | 100 (4/4) |
| P259G | Shell | + | 0 (0/25) |
| P259A | Shell | + | 93 (14/15) |
| P262G | Hinge | + | 100 (5/5) |
| P262A | Hinge | + | 100 (5/5) |
| T292A | Protrusion | + | 100 (5/5) |
| D293A | Protrusion | − | Not tested |
| W296A | Protrusion | − | Not tested |
| Q297A | Protrusion | + | 100 (5/5) |
| S338A | Protrusion | + | 100 (5/5) |
| S338F | Protrusion | − | Not tested |
| S338T | Protrusion | − | Not tested |
| S339G | Protrusion | + | 100 (5/5) |
| L353A | Protrusion | − | Not tested |
a
Data in parentheses represent the number of assays where CNV was transmitted by the zoospores/the total number of times the assay was performed for that mutant.