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. 2013 Nov;87(22):12102–12109. doi: 10.1128/JVI.02241-13

Fig 1.

Fig 1

Histologic analyses of IL-2 infusion in HSV-1-infected mouse CNS. Female C57BL/6 mice were treated by continuous infusion of purified mIL-2 administered via an Alzet microosmotic pump (1 μg/24 h) over successive 14-day periods. The pumps were inserted i.c. into the back of the skull of the mice through a small skin incision, and IL-2 was delivered into the cerebral ventricles using the Alzet brain infusion kit 1. Wound edges were repaired with surgical clips. Most animals began to regain weight 3 to 4 days after surgery. Osmotic pumps were loaded under sterile conditions with 30 μg of mIL-2 (Peprotech) in 100 μl of PBS. Two weeks after IL-2 infusion, some mice were ocularly infected with 2 × 105 PFU/eye of HSV-1 strain McKrae. Control mice were mock infected with a similar volume of medium without HSV-1 infection. wt control mice were similarly infected with HSV-1. Mice were sacrificed at 14 days p.i., and CNS tissues were stained with LFB. Arrows, areas of demyelination. (A) Results obtained when the pump was inserted i.c.; (B) results obtained when the pump was inserted in the side.