Fig 3.
CNS demyelination in mice injected with different forms of IL-2 DNA. (A) Schematic representation of the IL-2 constructs used for DNA injection. Full-length IL-2 is shown at the top. IL-2A and IL-2B, the 1st and 2nd halves of full-length IL-2, respectively; IL-2A1, the 1st half of IL-2A; IL-2A2, the 2nd half of IL-2A. All constructs have an ATG and a termination codon (TAA) and were inserted into pVR-1055, as described in the legend to Fig. 2. (B) Demyelination in injected mice. Female C57BL/6 mice were injected 3 times with 100 μg of each construct. Seven days after the 3rd immunization, mice were ocularly infected with 2 × 105 PFU/eye of HSV-1 strain McKrae. Mice were sacrificed at 14 days p.i., and CNS tissues were harvested and stained with LFB, as described in the legend to Fig. 1. Arrows, areas of demyelination.