Fig 6.

Virions are packed against VFs during TB reversion. (A) The confocal projection shows that unstressed protoplasts contain mostly normal VFs (white frame) characterized by colocalization of the P4 and P6 label. The insets present single optical sections of the VF delineated by the white frame in the main picture. The arrow points to a TB, identified by its characteristic rounded shape with a cortex rich in P4 and by lack of P6 label. (B) Confocal projection showing that virions (red label) accumulate close to VFs (yellow merge of the P4 and P6 label) in reverting protoplasts. The insets present separated channels of a single optical section of the VF marked by the white frame in the confocal projection. The arrows in the insets point to P4 label adjoining a VF. The red-labeled inclusions devoid of adjacent P6 label (arrowheads in the main picture) probably correspond to reforming TBs that are characterized by the absence of P6 or to virion aggregates. (C) Confocal projection of a wild-type CaMV-infected protoplast transfected with DAPI-labeled virions in blue and P6 immunolabeled in green. The inset shows a single optical section of the VF delineated in the projection. It is apparent that the extrageneously supplied virions colocalize with VFs. Scale bar, 5 μm for all.