Table 3.
Forward primer sequences used for custom small RNA real-time PCR assays
| Mature microRNA | Primer sequencesa |
|---|---|
| mir-K12-2 | AACUGUAGUCCGGGUCGAUCUG (wt) |
| AACUGUAGUCCGGGUAGAUCUG (v) | |
| mir-K12-4 | AGCUAAACCGCAGUACUCUAGG |
| mir-K12-5 | UAGGAUGCCUGGAACUUGCCGG |
| mir-K12-6 | UGAUGGUUUUCGGGCUGUUGAG (wt) |
| UGAUGGUUUUCGGGCUGUUGAA (v) | |
| mir-K12-7 | UGAUCCCAUGUUGCUGGCGC |
| mir-K12-9 | ACCCAGCUGCGUAAACCCCGCU (wt) |
| AUCCAG-CUAGGUAUACCCAGGU (v) | |
| mir-K12-10 | UAGUGUUGUCCCCCCGAGUGGC |
a
Boldface nucleotides indicate SNPs. Hyphen in sequence for mir-K12-9 represents a deletion.