Fig 2.

LANA and LANA deletion mutants associate with mitotic chromosomes, with preferential localization to areas near centromeres and telomeres. BJAB cells alone and BJAB cells stably expressing different LANA mutants were arrested in metaphase with colcemid and were analyzed for LANA localization by confocal microscopy. LANA (green) was detected with an antibody against LANA or against the T7 epitope tag. Chromosomes were counterstained with propidium iodide (red). The overlay of green and red results in yellow. White arrowheads and arrows indicate pericentromeric and peritelomeric localization of LANA, respectively. Brightness and contrast were similarly adjusted for the panels, and individual panels were uniformly adjusted using Adobe Photoshop. Magnification, ×630.