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. 2013 Nov;87(22):12270–12283. doi: 10.1128/JVI.01243-13

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Fig 3 — Deletion of internal regions of LANA does not abolish TR DNA binding as assessed by EMSA. A radiolabeled TR DNA probe was incubated with rabbit reticulocyte lysate (RRL) (lanes 1 and 7) or similar amounts (as detected by Western blotting) of in vitro-translated LANA (lanes 2 and 8), LANAΔ465-497 (lane 3), LANAΔ33-273 (lane 4), LANAΔ33-495 (lane 5), LANAΔ465-929 (lane 6), or LANAΔ264-929 (lane 9). After incubation, complexes were resolved on a nondenaturing polyacrylamide gel. Brightness and contrast were uniformly adjusted with Adobe Photoshop. All panels are from the same gel. Arrows indicate LANA-TR or mutated LANA-TR complexes. O, gel origin.