Fig 4.
Deletion of internal LANA regions does not reduce the half-life of LANA. Cells were treated with cycloheximide to arrest protein synthesis, and LANA levels were assessed by immunoblotting. (A) Results for BCBL-1 cells (∼1.5 × 105 cells loaded per lane) and for Louckes cells (∼3 × 105 cells loaded per lane) transfected with LANA, LANAΔ33-273, LANAΔ33-495, LANAΔ465-929, LANAΔ332-929, LANAΔ264-929, LANAΔ33-888, LANAΔ33-929, or LANAΔ33-949 are shown at time zero and at serial time points after cycloheximide arrest. Untransfected cells (not transf.) show a comigrating cross-reactive band with LANAΔ264-929. LANA was detected with affinity-purified antibody directed against the C-terminal region of LANA. Tubulin immunoblots are shown below each panel. (B) c-Myc was detected in the same cells as those used for panel A at time zero and at serial time points in order to assess the evidence for effective cycloheximide arrest. Results for untransfected cells are also shown. Tubulin blots are shown below each panel.