Fig 8.

LANAΔ465-929 and LANAΔ332-929 exhibit deficiencies in limiting-dilution outgrowth after TR DNA transfection and G418 selection. (A) BJAB cells alone and BJAB cells stably expressing LANA or LANAΔ465-497 were transfected with plasmid p8TR and were seeded in 96-well plates at different cell concentrations. Well outgrowth was recorded at 20 days under G418 selection. Values are averages for 3 experiments; error bars indicate standard deviations. (B) Limiting-dilution assay for BJAB cells alone and BJAB cells stably expressing LANA, LANAΔ465-929, or LANAΔ332-929 after p8TR transfection and G418 selection. (C) Cells stably expressing LANAΔ465-929 or LANAΔ332-929 were assessed for rates of G418-resistant outgrowth after plating at different cell concentrations. Shown are the numbers of days necessary for sufficient G418-resistant outgrowth to allow the expansion of at least half of the positive wells in 96-well plates to 24-well plates.