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Typical MS2 spectra of O-glycopeptides: A. MS2 spectrum of HPX glycopeptide Hex-GalNAc (m/z 770.8, +4) with dominant de-glycosylated peptide fragment ion (m/z 906.12, +3) and subdominant y and b peptide fragment ions; B. MS2 spectrum of SHBG glycopeptide Neu5Ac-Hex-GalNAc (m/z 623.4, +3) with dominant de-glycosylated peptide fragment ion (m/z 612.3, +2), neuraminic acid oxonium ion (m/z 274.06), subdominant peptide fragment ion and peptide-attached glycan fragment ions.
