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. Author manuscript; available in PMC: 2014 Sep 15.
Published in final edited form as: Toxicology. 2013 Jul 8;311(3):10.1016/j.tox.2013.06.013. doi: 10.1016/j.tox.2013.06.013

Figure 1. Effect of p53 on NM-induced epidermal thickness and percentage dead epidermis.

Figure 1

Dorsal skin of WT and p53+/− and p53−/− SKH-1 hairless mice was exposed topically to 3.2 mg NM for 24 and 72 h. Thereafter, mice were sacrificed and skin tissues were collected and processed as detailed under material and methods. Whole cell lysates were prepared from the skin and subjected to western immunoblot analysis for p53 Ser15 as detailed under Materials and Methods (A). Protein loading was determined by stripping and reprobing the membrane with β-Actin antibody (A). 5µm thick skin sections were processed for H&E staining and analyzed for epidermal thickness and percentage dead epidermis. Epidermal thickness after 24 h of NM exposure is shown in representative pictures (B), which was quantified (C) as detailed in materials and methods. Percentage dead epidermis shown in representative pictures (D) was quantified at 72 h of NM exposure (E). Data are presented as mean ± SEM of four animals in NM-exposed groups and three in respective controls. *, p < 0.05 as compared to controls. e, epidermis; d, dermis; blue arrows, dead epidermis. Black scale bar = 100 µm.