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. Author manuscript; available in PMC: 2013 Oct 25.

Published in final edited form as: J Cancer Ther. 2013 May;4(3):10.4236/jct.2013.43094. doi: 10.4236/jct.2013.43094

MALAT-1 promoter activity and transcript levels correlate with DNA tumor virus oncogene expression. A. Representative data showing increased MALAT-1 promoter activity 48 hours after HPV16 E6 transfection and not with HPV16 E7. B. Higher levels of MALAT-1 transcription, expressed as n-fold change in BK polyomavirus-infected human parotid gland (HSG) cells. Levels in mock samples were arbitrarily set to 1. Left. Northern blot showing a four-fold increase in MALAT-1 transcript levels in Vero cells transfected with BKV DNA. Right C. Semi-quantitative real-time RT-PCR shows amplified MALAT-1 cDNA bands from BKV-transfected Vero cells at stated times after transfection. D. Quantitative real-time RT-PCR shows increased MALAT-1 transcript levels in BKV-transfected Vero cells at stated times after transfection. Expression values presented as the change (n-fold) in MALAT-1 transcript levels, with levels at time 0 arbitrarily set to 1. E. Quantitative real-time RT-PCR showing BKV Tag and p21 transcript levels over stated times after BKV infection. F. MALAT-1 promoter activity in BKV-infected and uninfected Vero cells at 48 and 72 hours after transfection. Top. BKV T ag protein (top) detected in Vero cells infected with BK virus and transfected with the MALAT-1 promoter plasmid. G. BKV T ag up-regulated MALAT-1 promoter activity regardless of T ag pRb binding status in Vero cells containing wild-type p53 compare to positive and negative controls, pSEAPc and pSEAPb, respectively. H. Neither wild-type BKV T ag nor T ag pRb binding mutant enhanced MALAT-1 transcript levels in human salivary gland cells containing mutant p53 expression.

MALAT-1 promoter activity and transcript levels correlate with DNA tumor virus oncogene expression. A. Representative data showing increased MALAT-1 promoter activity 48 hours after HPV16 E6 transfection and not with HPV16 E7. B. Higher levels of MALAT-1 transcription, expressed as n-fold change in BK polyomavirus-infected human parotid gland (HSG) cells. Levels in mock samples were arbitrarily set to 1. Left. Northern blot showing a four-fold increase in MALAT-1 transcript levels in Vero cells transfected with BKV DNA. Right C. Semi-quantitative real-time RT-PCR shows amplified MALAT-1 cDNA bands from BKV-transfected Vero cells at stated times after transfection. D. Quantitative real-time RT-PCR shows increased MALAT-1 transcript levels in BKV-transfected Vero cells at stated times after transfection. Expression values presented as the change (n-fold) in MALAT-1 transcript levels, with levels at time 0 arbitrarily set to 1. E. Quantitative real-time RT-PCR showing BKV Tag and p21 transcript levels over stated times after BKV infection. F. MALAT-1 promoter activity in BKV-infected and uninfected Vero cells at 48 and 72 hours after transfection. Top. BKV T ag protein (top) detected in Vero cells infected with BK virus and transfected with the MALAT-1 promoter plasmid. G. BKV T ag up-regulated MALAT-1 promoter activity regardless of T ag pRb binding status in Vero cells containing wild-type p53 compare to positive and negative controls, pSEAPc and pSEAPb, respectively. H. Neither wild-type BKV T ag nor T ag pRb binding mutant enhanced MALAT-1 transcript levels in human salivary gland cells containing mutant p53 expression.