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. 2013 Jul 16;21(10):1938–1949. doi: 10.1038/mt.2013.141

Figure 1.

Figure 1

Comparison of eGFP expression from myeloid promoters. (a) pCCL LVs containing human CD18, CD11b, and PGK promoters driving eGFP expression. (b) WT bone marrow was lineage depleted and the enriched stem cell population transduced with LV-hCD18-eGFP, LV-hCD11b-eGFP, or LV-hPGK-eGFP at an MOI of 40–70 and transplanted into myeloablated WT mice. n = 8–10 (c) Donor and eGFP+ chimerism was determined 24 weeks post-transplantation. The vector copy number (VCN) was determined in WBCs. (d–e) Mean fluorescent intensity of eGFP expression was determined for all leukocytes and for CD11b+, CD19+, and CD3+ cells 24 weeks post-transplantation. (f–h) Mice with most equivalent copy numbers (VCN of 2.3–4.1) in WBCs were further compared. Error bars represent the SEM. Significant differences between the two groups marked with a line are demonstrated with *P < 0.05; **P < 0.01; and ***P < 0.001. CMV, cytomegalovirus; eGFP, enhanced green fluorescent protein; hPGK, human phosphoglycerate kinase; LV, lentiviral vector; MOI, multiplicity of infection; ns, nonsignificant; WBC, white blood cell; WT, wild-type.