Figure 5.

Viral replication and cytopathic effects of VSV-FH and the parental viruses. (a) Specificity of receptor usage by VSV-FH. A panel of CHO cells (2.5 × 10⁵ cells), expressing specific MV receptors, was infected with MV (MOI = 0.1), VSV (MOI = 0.01), or VSV-FH (MOI = 0.01) and stained with crystal violet at 24 hours (VSV and VSV-FH) or 48 hours (MV). (b) Viral progeny production over time in Vero cells. Cells were infected with a MOI of 0.1 (MV) or 0.00001 (VSV or VSV-FH), cells and supernatant were harvested at the indicated times and the amount of infectious particles was determined by TCID₅₀ titrations (mean ± SD, n = 3). (c) Cytopathic effect at 72 hours in cells previously infected with MV, VSV-FH, or VSV at the indicated multiplicities of infection. Cells were infected at different MOIs with the indicated viruses. At 3 days after infection, cells were fixed and stained with crystal violet. MOI, multiplicity of infection; MV, measles virus; VSV, vesicular stomatitis virus.