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. Author manuscript; available in PMC: 2014 Feb 13.
Published in final edited form as: Cell Host Microbe. 2013 Feb 13;13(2):10.1016/j.chom.2013.01.009. doi: 10.1016/j.chom.2013.01.009

Figure 1. The pUL25 Capsid Protein Unmasks VP1/2 Nuclear Membrane Localization.

Figure 1

(A) Schematic representation of seven regions of VP1/2. Regions 2 and 6 are proline rich (black). Position of the capsid binding domain (CBD) consisting of amino acids 3034–3095 is indicated. Amino acid positions based on GenBank JF797219.1 are indicated above the schematic (Szpara et al., 2011).

(B) Transiently expressed mCherry-VP1/2 or GFP-pUL25 were diffuse in Vero cells, with mCherry-VP1/2 often enriched in the nucleus.

(C) Coexpressed mCherry-VP1/2 and GFP-pUL25 were enriched in the perinuclear region and nuclear rim.

(D) VP1/2 localized to the nuclear rim in the absence of pUL25 when the capsid/pUL25 binding domain was removed (ΔR7). Images were captured 18–24 hr posttransfection (hpt). Scale bars are 20 µm.