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. Author manuscript; available in PMC: 2014 Feb 13.
Published in final edited form as: Cell Host Microbe. 2013 Feb 13;13(2):10.1016/j.chom.2013.01.009. doi: 10.1016/j.chom.2013.01.009

Figure 5. Capsid Transport Dynamics in Sensory Axons.

Figure 5

(A–F) DRG explants were infected with the WT, MUT, or REP viruses at 7.0 3 106 pfu/coverslip. During the first hour postinfection, moving fluorescent capsids were imaged at 10 frames per second. (A) DRG neuron infected with RFP-capsid tagged virus is illustrated at top. Middle panel is the first frame from a time-lapse recording with the corresponding kymograph aligned below (d, distance; t, time). Scale bar, 10 mm. (B) Profile of capsid retrograde transport velocities. Each data point is an average velocity of a capsid run (uninterrupted period of unidirectional transport). Data were combined from three replicate experiments (n > 80 capsid recordings per experiment). (C) Profile of run lengths. Analyses in (B) and (C) are from the same data set. (D and E) Average velocity and length of retrograde runs longer than 0.5 mm based on data presented in (B) and (C). (F) Frequency of anterograde runs ≥ 0.5 mm per particle. Error bars are SEM based on three replicate experiments per sample. p values were determined by one-way analysis of variance and a post hoc Tukey’s test.