Table 1.

Kinetics parameters obtained for the oxidation of the different substrates

Protein	Substrate	Km (mM)	kcat (min−1)	kcat/Km (M−1 min−1)
di-Fe(III)-DF3	3,5-DTBCa	2.09 ± 0.31	13.20 ± 1.21	6,315
	4-APb	1.97 ± 0.27	2.72 ± 0.19	1,380
	PPDc	8.87 ± 2.58	0.73 ± 0.03	83
	OPDd		Not detected
G4-DFtet	4-APb	0.83 ± 0.06	1.30 ± 0.10	1,540

All experiments were performed in 100 mM HEPES/100 mM NaCl buffer (pH 7.0). Kinetic parameters represent mean values ± s.d., calculated by two independent measurements.

^a3,5-di-tert-butylcatechol.

^b4-aminophenol.

^cp-phenylendiamine.

^do-phenylendiamine.