Table 1.

Serum neutralizing antibody (PRNT) responses and RVFV IgG OD₄₁₄ values of calves in the Phase I studies that were inoculated s.c. with either 1×10¹, 1×10², 1×10³, 1×10⁴, 1×10⁵ or 1×10⁷ PFU of arMP-12ΔNSm21/384. No statistically significant differences were calculated between the dosage treatments for PRNT response. The difference in IgG response between the 1×10¹ PFU group and the 1×10⁵ PFU group was statistically significant (p<0.05). The negative cutoff value for IgG was 0.22.

Days Post Inoculation
Vaccine Dose: 1 × 101 pfu
No.†	0		7		10		14		21		28		35		49
	PRNT‡	IgG‡‡	PRNT	IgG	PRNT	IgG	PRNT	IgG	PRNT	IgG	PRNT	IgG	PRNT	IgG	PRNT	IgG
89	<1:10	0.02	<1:10	0.00	<1:10	0.02	40	0.04	80	0.09	80	0.07	80	0.13	160	0.19
90	<1:10	−0.03	<1:10	−0.03	<1:10	0.00	<1:10	0.02	20	0.12	80	0.23	20	0.21	80	0.45
91	<1:10	0.04	<1:10	0.03	<1:10	0.02	<1:10	0.06	<1:10	0.10	<1:10	0.00	<1:10	−0.03	10	0.01
Vaccine Dose: 1 × 102 pfu
92	<1:10	0.14	<1:10	0.07	<1:10	0.07	20	0.09	20	0.10	20	0.16	20	0.06	80	0.06
93	<1:10	0.20	<1:10	0.10	<1:10	0.29	1280	0.32	640	0.45	320	0.34	320	0.79	1280	0.74
94	<1:10	0.10	<1:10	−0.13	<1:10	−0.01	80	0.02	20	−0.03	80	0.12	40	0.18	80	0.24
Vaccine Dose: 1 × 103 pfu
95	<1:10	0.16	<1:10	0.24	20	0.33	80	0.57	320	0.83	320	0.96	320	0.80	1280	1.24
96	<1:10	0.04	<1:10	0.14	20	0.10	160	0.52	80	0.37	80	0.34	160	1.13	320	0.82
97	<1:10	0.03	<1:10	0.03	<1:10	0.06	80	0.37	320	0.53	320	0.82	320	0.98	640	1.18
Vaccine Dose: 1 × 104 pfu
101	<1:10	0.08	<1:10	0.07	<1:10	0.28	80	0.22	20	0.14	20	0.17	20	0.19	20	0.17
102	<1:10	−0.19	<1:10	0.14	80	0.36	80	0.59	320	0.52	320	0.89	320	1.25	320	1.47
103	<1:10	0.01	<1:10	−0.13	<1:10	−0.01	320	0.13	80	0.29	80	0.58	320	0.69	320	0.89
104	<1:10	−0.21	<1:10	−0.03	20	0.07	20	0.09	20	0.09	20	0.18	20	0.17	80	0.26
Vaccine Dose: 1 × 105 pfu
105	<1:10	0.05	<1:10	0.09	80	0.26	80	0.46	80	1.02	160	1.23	640	2.18	1280	2.38
106	<1:10	0.05	<1:10	0.24	20	0.43	320	0.63	320	1.03	320	1.37	1280	1.92	1280	2.46
107	<1:10	0.04	<1:10	0.14	320	0.49	320	0.65	80	1.06	80	1.46	320	1.71	320	2.04
108	<1:10	0.04	<1:10	0.52	80	0.77	80	1.15	320	1.50	80	1.90	1280	2.19	1280	2.37
Vaccine Dose: 1 × 107 pfu
98	<1:10	−0.13	<1:10	0.18	20	0.29	40	0.62	80	0.55	80	0.93	80	0.67	320	0.94
99	<1:10	0.09	20	0.14	80	0.29	160	0.42	80	0.66	320	1.51	160	1.40	320	1.59
100	<1:10	−0.02	20	0.24	80	0.49	160	0.73	80	0.59	320	1.03	160	1.17	320	1.16

^†Calf ear tag number.

^‡Data are expressed as the reciprocal of plaque-reduction neutralization 80% antibody titer. Minimum detection limit of the assay is 1:10.

^‡‡Adjusted OD₄₁₄values of sera diluted 1:100