Table 2.
The size and abundance of VGLUT1+ and VGLUT2+ synaptic terminals in striatum of WT and heterozygous Q140 knock-in Huntington disease mice, as determined by quantification of EM images, are shown. The columns show the number of Q140 and WT animals analyzed per age group, the mean CAG repeat length for Q140 mice per age group, and the size or abundance of VGLUT1 axospinous, VGLUT2 axospinous, VGLUT1 axodendritic, and VGLUT2 axodendritic terminals. Parametric t-tests were used to evaluate differences between WT and Q140 mice within each of the three age groups examined for each of the parameters measured, with the exceptions that a Mann-Whitney U nonparametric t-test was performed for VGLUT1 axospinous and axodendritic terminal size at one month, for VGLUT1 axospinous terminal spatial frequency at one month, and for VGLUT2 axospinous terminal spatial frequency at 1 and 12 months, since they were not normally distributed.
| Age | Animals | Q140 CAG |
VGLUT1 Axospinous Terminal Diameter |
VGLUT2 Axospinous Terminal Diameter |
VGLUT1 Axodendritic Terminal Diameter |
VGLUT2 Axodendritic Terminal Diameter |
Results Shown in |
|---|---|---|---|---|---|---|---|
| 1 month | 7 WT, 7 Q140 | 126.8 | WT=0.492 Q140=0.485 |
WT=0.514 Q140=0.521 |
WT=0.565 Q140=0.572 |
WT=0.615 Q140=0.566 |
in µm |
| 4 month | 5 WT, 5 Q140 | 128.6 | WT=0.604 Q140=0.593 |
WT=0.623 Q140=0.603 |
WT=0.644 Q140=0.661 |
WT=0.671 Q140=0.641 |
in µm |
| 12 month | 5 WT, 5 Q140 | 135.0 | WT=0.608 Q140=0.628 |
WT=0.656 Q140=0.660 |
WT=0.902 Q140=0.696 |
WT=0.672 Q140=0.649 |
in µm |
| Age | Animals |
Q140 CAG |
VGLUT1 Axospinous Terminal Abundance |
VGLUT2 Axospinous Terminal Abundance |
VGLUT1 Axodendritic Terminal Abundance |
VGLUT2 Axodendritic Terminal Abundance |
Results Shown as |
| 1 month | 7 WT, 7 Q140 | 126.8 | WT=0.1520±0.0150 Q140=0.1461±0.0132 |
WT=0.1055±0.0120 Q140=0.0846±0.0065 |
WT=0.0114±0.0039 Q140=0.0144±0.0046 |
WT=0.0260±0.0030 Q140=0.0149±0.0023 |
per µm2 |
| 4 month | 5 WT, 5 Q140 | 128.6 | WT=0.1295±0.0114 Q140=0.1402±0.0115 |
WT=0.0902±0.0061 Q140=0.0730±0.0065 |
WT=0.0059±0.0020 Q140=0.0096±0.0041 |
WT=0.0127±0.0021 Q140=0.0180±0.0024 |
per µm2 |
| 12 month | 5 WT, 5 Q140 | 135.0 |
WT=0.1444±0.0077 Q140=0.1050±0.0055 |
WT=0.1030±0.0105 Q140=0.0851±0.0033 |
WT=0.0079±0.0033 Q140=0.0061±0.0014 |
WT=0.0111±0.0014 Q140=0.0144±0.0026 |
per µm2 |
Mean ± Standard Deviation,
Bold = p<0.05,
Bold & ital = 0.5–0.8 µm range significant at p<0.05 with Bonferroni correction for multiple comparisons