Nomenclature and CD59 surface expression of iPSC lines. (A):
PIG-A was targeted with zinc finger nucleases in parental hFib2-iPS5 cells to establish the PIG-A-null induced pluripotent stem cell line hFib2-iPS5-PIG-A HR (FPHR). FPHR cells were then transduced with a lentiviral vector containing the full-length PIG-A cDNA to establish the FPHR-PIG-A cell line. (B): Flow cytometric analysis for CD59 expression in hFib2-iPS5, FPHR, and FPHR-PIG-A cells (hFib2-iPS5 cells were used for the isotype control). Abbreviations: APC, allophycocyanin; iPS, induced pluripotent stem cell; ZFN, zinc finger nuclease.