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. 2013 Oct 10;2(11):819–829. doi: 10.5966/sctm.2013-0069

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©AlphaMed Press 1066-5099/2013/$20.00/0

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Figure 2. — FPHR cells are unable to differentiate to hematopoietic stem cell/progenitors. Embryoid body (EB) generation was performed in hFib2-iPS5, FPHR, and FPHR-PIG-A cells using forced aggregation. (A): Morphology of the EBs from hFib2-iPS5, FPHR, and FPHR-PIG-A cells. Blood cells were observed surrounding hFib2-iPS5 (left) and FPHR-PIG-A (right) EBs but not FPHR EBs (middle). Morphology of the EBs from hFib2-iPS5, FPHR, and FPHR-PIG-A. Scale bars = 200 μm. (B): The expression of hematopoietic markers CD34 and CD45 is shown for hFib2-iPS5 cells (left), FPHR cells (middle), and FPHR-PIG-A cells (right). (C): Enumeration of CFUs from hFib2-iPS5, FPHR, and FPHR-PIG-A cells. Error bars represent the SD based on three experiments. No colonies formed from the FPHR cells; there was no significant difference in total CFU colonies between hFib2-iPS5 and FPHR-PIG-A (p > .05) cell types. Abbreviations: APC, allophycocyanin; CFU, colony-forming unit; CFU-E, colony-forming unit-erythroid; CFU-GM, colony-forming unit-granulocyte-macrophage; CFU-M, colony-forming unit-macrophage; iPS, induced pluripotent stem cell; PE, phycoerythrin.