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. 2013 Oct 10;2(11):819–829. doi: 10.5966/sctm.2013-0069

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©AlphaMed Press 1066-5099/2013/$20.00/0

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Figure 3. — Time course of early mesodermal markers and hematopoietic stem cell markers during EB differentiation on days 3, 7, and 10. The expression of CD56, KDR/VEGFR2, and CD34 was analyzed in hFib2-iPS5 (○, solid line), FPHR (▴, solid line), and FPHR-PIG-A (♦, dashed line). Error bars represent the SD based on six experiments. (A): CD56 expression was significantly increased in hFib2-iPS5 and FPHR-PIG-A but not in FPHR cells during EB differentiation at days 3 and 7. (B): KDR/VEGFR2 expression was significantly elevated in hFib2-iPS5 and FPHR-PIG-A compared with FPHR cells in day 3 and 7 EBs. (C): CD34 expression was significantly increased at day 7 and highly significantly increased for day 10 EBs in hFib2-iPS5 and FPHR-PIG-A cells compared with FPHR cells. *, p < .05; **, p < .01. Abbreviations: EB, embryoid body; iPS, induced pluripotent stem cell.