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The morphology of cells during neural induction. (A): Workflow of NSC derivation from hPSCs. (B): hPSCs cultured in feeder-free conditions at day 1 of splitting with 10%–15% of confluence. (C–G): The morphology of cells at days 1 (C), 2 (D), 4 (E), 6 (F), and 7 (G) after neural induction. Inset in (G) shows passage 0 NSCs at day 1 of replating after dissociation. Scale bar = 100 μm. Abbreviations: hPSC, human pluripotent stem cell; NSC, neural stem cell.
