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. 2013 Oct 10;2(11):862–870. doi: 10.5966/sctm.2013-0080

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©AlphaMed Press 1066-5099/2013/$20.00/0

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Figure 7. — Heat map. (A): Expression of NSC-related genes and default regional identity of the sample population shown by a heat map generated by the R software. NSCs from pluripotent stem cell (PSC) origin expressed most of the NSC-related genes that are also expressed by fNSCs. Primitive NSCs expressed genes related to ventral hindbrain as in default. The gene expression data are log2 transformed. (B): Expression of select non-neural genes and identity of the sample population shown by heat map. NSCs from PSCs at passage 0 (P0) (pNSCp0) and expanded NSCs to P6 (pNSCp6), rdNSCs, and human fNSCs are included for comparison. The gene expression data are normalized (i.e., all genes have a mean of 0 and an SD of 1). Abbreviations: fNSC, fetal cortex-derived neural stem cell; NSC, neural stem cell; pNSCp0, NSCs on differentiation day 7 in neural induction medium; pNSCp6, pNSCp0 passaged six times; rdNSC, rosette-derived neural stem cell.