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. 2013 Oct 25;8(10):e77994. doi: 10.1371/journal.pone.0077994

Figure 5. Releasing Brd4 from chromatin by JQ1(+) abolishes the E2-Brd4 interaction on mitotic chromosomes.

Figure 5

(A) C33A cells were co-transfected with VN-Brd4 and VC-16E2 and treated with 500 nM JQ1(-) or JQ1(+) at 24h post transfection. Forty-eight hours post-transfection, cells were either fixed immediately (before wash) or washed several times and cultured for the times indicated on the right panel. All cells were fixed and stained with anti-FLAG antibody (red) and DAPI. (B) C33A cells were co-transfected with VN-Brd4 and VC-16E2. Forty-eight hours post-transfection, cells were treated with 500 nM JQ1(-) or JQ1(+) for 1h. The cells were then fixed and stained with anti-FLAG antibody (red) and DAPI. Merge 1 is a merge of the BIFC and DAPI panels and Merge 2 is a merge of BIFC, FLAG and DAPI panels. Bar, 5 μm. The white arrow marks a BiFC focus superimposed on a mitotic chromosome.