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. 2013 Oct 25;8(10):e79312. doi: 10.1371/journal.pone.0079312

Figure 1. Estradiol-induced expression of ATML1-SRDX.

Figure 1

(A) Representation of an estradiol-inducible gene expression system used to express ATML1-SRDX. XVE (LexA DNA-binding domain/VP16 activation domain/estrogen receptor) was expressed under the control of the RPS5A promoter (proRPS5A) [8,17]. After treatment with estradiol, XVE binds to the LexA binding site (OlexA) and activates ATML1-SRDX expression. For simplicity, hpt (conferring hygromycin resistance) was omitted from this figure. T, transcriptional terminator. (B) ATML1-SRDX expression in seven-day-old seedlings of estradiol-inducible lines (#1, #3, and #10) and the wild type (WT) grown in the absence (−) or presence (+) of 10 μM estradiol. Data were normalized to the amount of PP2A [20]. Values are the mean ± SEM from three biological replicates. Asterisks indicate a statistically significant difference relative to the wild type (unpaired two-tailed t-test; p < 0.05). Note that transcripts of ATML1-SRDX were not detected in the wild type and were almost absent in lines 1, 3, and 10 without estradiol treatment.