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. 2013 Oct 25;8(10):e79312. doi: 10.1371/journal.pone.0079312

Figure 2. Phenotypes of ATML1-SRDX lines.

Figure 2

(A–C) Seven-day-old seedlings of the wild type (A), a type I ATML1-SRDX line (B), and a type II ATML1-SRDX line (C) grown on MS-phytagel plates containing 10 μM estradiol. The arrow indicates fused leaves formed at the shoot apex. (D–F) Seven-day-old seedlings of the wild type (D), a type I ATML1-SRDX line (E), and a type II ATML1-SRDX line (F) grown with 10 μM estradiol and stained with toluidine blue (blue color). (G–I) Longitudinal sections of seven-day-old wild-type (G), type I ATML1-SRDX (H), and type II ATML1-SRDX seedlings (I) grown with 10 μM estradiol and stained with toluidine blue. The arrow in I indicates a leaf primordium. In Figure 2G–2I, toluidine blue staining was performed after sectioning to visualize the cell boundaries. (J) A 16-day-old seedling of the wild type grown with 0.1 μM estradiol. (K) Fused leaf development (arrows) in a type I ATML1-SRDX line grown with 0.1 μM estradiol for 16 days. (L) Magnified view of the fused leaves in K. The arrowhead indicates a junction of the fused leaves. (M) A 14-day-old seedling of the wild type grown with 10 μM estradiol. (N) Fused leaf formation in a type II ATML1-SRDX line grown with 10 μM estradiol for 14 days. co, cotyledon. (O) An eight-day-old seedling of a type II ATML1-SRDX line grown with 10 μM estradiol. Droplets of water were seen on the surface of the seedling. (P, Q) 14-day-old seedlings of the wild type (P) and a type I ATML1-SRDX line (Q) germinated on estradiol-free MS plates and transferred to 10 μM estradiol-containing plates after 5 days. Filamentous protrusions were formed on the surface of the leaves. (R) Calli formation from the cut end of a wild-type Columbia cotyledon. Scale bars, 1 mm for A–F, N, O, Q, R; 1 cm for J, M, P; 5 mm for K, L; and 200 μm for G–I.